Solid Phase Extraction

 

 

Reverse Phase SPE Cartridges, Non-Polar

Non-Silica SPE Cartridges

Normal Phase SPE Cartridges, Polar

Ion Exchange SPE Cartridges

 

 

Solid Phase Extraction cartridges provide fast and efficient sample clean-up and concentration prior to analysis. Our cartridges use medical grade polypropylene tubes, porous polyethylene frits, and highly reproducible sorbents. Cartridges are designed with both manual and automated uses in mind. All of our SPE syringe cartridges are equipped with male Luer tips, for versatility in connections and processing.

 

Choosing the Proper Packing Type:

 

Reversed Phase - C18, C8, C4, C2 and Phenyl bonded phases are available and offer a variety of selectivities. Non-polar to moderately polar compounds in a polar matrix are reatined by reversed-phase sorbents. Reversed-phase packings require conditioning with an organic solvent followed by an aqueous solvent prior to use. Elution of moderately polar compunds is often accomplished with mid-polarity solvents while non-polar compounds require less polar elovents.

 

Normal Phase - Silica, Florsil, Amino, Cyano, Diol and Alumina are ususally used to retain polar compounds from non-polar matrices. Conditioning is carried out with a non-polar solvent. Elution is accomplished with more polar solvents. Compunds with pH basic functional groups are readily retianed by silica. However, very polar compounds such as carbohydrates can be irreversibly retained on silica surface. In this case, a bonded and mid-polarity phase such as Diol or Amino is a better choice.

 

Ion Exchange - Strong anion (SAX) and cation (SCX) exchangers are available. Anions andcations are retained on the corresponding resin by exchanging the anion or cation in the buffers, 0.1 M - 0.5 M, or by changing the pH of elution solvent such that the sample compound is no longer charged. These resins are silica based and lose some exchange capacity with increasing organic solvent in the sample.